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BAB Bs200Pro / Bs200Doc İle Yapılan Bazı Yayınlar |
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Radiation Physics and Chemistry (02 June 2009)
A simple technique of microgel electrophoresis of single
cells (DNA Comet Assay) was used to detect DNA comets in
irradiated quail meat samples. Obtained DNA comets were
evaluated by both photomicrographic and image analysis.Quail
meat samples were exposed to radiation doses of 0.52, 1.05,
1.45, 2.00, 2.92 and 4.00 kGy in gamma cell (Gammacell 60 Co,
dose rate 1.31 kGy/h) covering the permissible limits for
enzymatic decay and stored at 2 o C. The cells isolated from
muscle (chest, thorax) in cold PBS were analysed using the DNA
comet assay on 1, 2, 3, 4, 7, 8 and 11 day post irradiation.
The cells were lysed between 2, 5 and 9 min in 2.5% SDS and
electrophroesis was carried out at a voltage of 2 V/cm for 2
min. After propidium iodide staining, the slides were
evaluated through a fluorescent microscope. In all irradiated
samples, fragmented DNA stretched towards the anode and
damaged cells appeared as a comet. All measurement data were
analyzed using BS 200 ProP with software image analysis (BS
200 ProP, BAB Imaging System, Ankara, Turkey). The density of
DNA in the tails increased with increasing radiation dose.
However, in non-irradiated samples, the large molecules of DNA
remained relatively intact and there was only minor or no
migration of DNA; the cells were round or had very short tails
only. The values of tail DNA %, tail length and tail moment
were significantly different and identical between 0.9 and 4.0
kGy dose exposure, and also among storage times on day 1, 4
and 8. In conclusion, DNA Comet Assay EN 13784 standard method
may be used for not only screening method for detection of
irradiated quail meat depending on storage time and condition
but also for the quantification of applied dose if it is
combined with image analysis.Image analysis may provide a
powerful tool for the evaluation of head and tail of comet
intensity related with applied doses.
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[PDF]
Dosya Biçimi: PDF/Adobe Acrobat - HTML
olarak görüntüle
mikroskopta 'Comet Görüntü işleme ve Analiz Programı
Sistemi' ( BAB Bs 200 Pro) programı kullanılarak değerlendirme
yapılmıştır. ...
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İLAÇ UYGULAMA PERFORMANSININ DEĞERLENDİRİLMESİNDE KALİTATİF VE
KANTİTATİF ANALİZ YÖNTEMLERİNİN İNCELENMESİ
Bahadır SAYINCI* Saim BASTABAN
Atatürk Üniversitesi Ziraat Fakültesi Tarım Makinaları Bölümü 25240 Erzurum
*e-mail: bsayinci@atauni.edu.tr
Geliş Tarihi: 20.10.2008 Kabul Tarihi: 10.03.2009
ÖZET: İlaçlama ünitelerinin uygulama performanslarını belirlemek için çeşitli kalitatif ve kantitatif yöntemler geliştirilmiştir. Uygulamalarda hata varyansını azaltmak için çok sayıda örneğin analizi yapıldığından, ölçüm yöntemlerinin pratik, kolay ve ekonomik olması arzu edilir. Kalitatif yöntemlerde ölçümler, görüntü işleme programlarıyla yapılmaktadır(Bs200Pro, BAB Ltd. Şti.,
TR). Bu yöntemde örnekleme materyali olarak suya duyarlı, yağa duyarlı kartlar ve kromekote kartları kullanılmakta ve neme duyarlı olduklarından uygun olmayan şartlarda renk değiştirerek görüntü işleme kaliteleri bozulmaktadır. Kantitatif yöntemlerde ise filtre kağıdı, petri kabı ve bitki yaprağı gibi örnekleme materyalleri kullanılmakta ve uygulamalarda püskürtme sıvısına pestisit yerine gıda boyası karıştırılmaktadır. Bu yöntemde yüzeyden yıkanan maddenin bozulmadan tamamen çözücüye karışması, kullanılan örnekleme materyalinin özelliğine bağlıdır. Ayrıca uygulamadan sonra yüzeyde tutunan madde, açık havada solar radyasyona maruz kaldığından bozulabilmekte ve çözeltinin konsantrasyonu değişebilmektedir. Bu çalışmada kalitatif ve kantitatif analiz yöntemlerinde görüntü işleme kalitesini, renk maddelerinin bozulmasını ve renk maddelerinin geri kazanımını etkileyen faktörler literatür bilgileri ışığında derlenmiştir.
Anahtar Sözcükler: Görüntü İşleme Analizi, Suya Duyarlı Kart, Renk Maddelerinde Bozulma, Geri Kazanım
INVESTIGATION OF QUALITATIVE AND QUANTITATIVE ANALYSIS METHODS IN EVALUATING
SPRAY APPLICATION PERFORMANCE
ABSTRACT: Various qualitative and quantitative methods were developed for the determination of spray application performance. These methods are desired to be practical, easy and economic because of analyzing lots of samples to decrease error variance. The measurements in quantitative methods were made with image processing software. Water-sensitive, oil-sensitive and kromekote cards are used as sampling materials and their image processing quality is spoiled by changing color at the unsuitable conditions because of their sensitivity to humidity. Sampling materials such a filter paper, petri dishes and plant leaf are used in quantitative methods and food dye is mixed to spray liquid instead of pesticide in the spray application. At this method, the mixing of dye completely washed from surface without degradation, depends on the properties of the used sampling material. On the other hand, dye deposited on the surface after the application may be degraded due to its explosion to solar radiation at the open atmosphere and this can change the concentration of solution. In this study, the factors affecting the recovery of color dyes, degradation of dye materials and image processing quality in qualitative and quantitative methods were reviewed regarding up to date literatures.
Keywords: Image Processing Analysis, Water Sensitive Paper, Degradation of Dye, Spray Recovery
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Tolga
GUVENC*, Murat YARIM, Mustafa Yavuz GULBAHAR and Yonca B. KABAK
Department
of Pathology, Faculty of Veterinary Medicine, University of
Ondokuz Mayis,
Kurupelit,
55139 Samsun, Turkey
(Received
2 April 2007; accepted 6 September 2007)
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Journal of Neurosurgical Anesthesiology. 19(3):161-165, July
2007.
Turgut, Namigar MD *; Turkmen, Aygen MD *; Gokkaya, Sumru MD
*; Hatiboglu, Mustafa Aziz MD +; Iplikcioglu, Ahmet Celal MD +;
Altan, Aysel MD *
Abstract:
We tested the hypothesis that 5 cm H2O of positive end-expiratory
pressure (PEEP) reduces the incidence of pneumocephalus in
patients who undergo spinal intradural tumor surgery. Fifty-three
ASA I to III patients who underwent thoracolumbar intradural tumor
surgery between the years 2003 and 2006 were included in this
study. All patients received propofol, fentanyl, and cisatracurium
for induction of the anesthesia. Maintenance was provided by
propofol infusion and, oxygen (50%) and air (50%). Group I (n=28)
did not receive PEEP whereas group II (n=25) received PEEP as 5 cm
H2O. Cranial computerized tomography was taken at 8 hours after
the surgery and cases were evaluated for pneumocephalus using BAB
Bs200ProP Image System software. Pneumocephalus areas between 0.03
and 4.24 cm2 were observed in 9 patients, 8 in group I and 1
patient in group II at the 8th postoperative hour, at various
localizations. There were no neurologic findings in other patients
except for 2 patients in group I who presented with headache and
mental status change. Although the cerebrospinal fluid leakage is
minimal, N2O is not used and the patients are well hydrated,
pneumocephalus with neurologic deficits may occur in patients
undergoing microsurgical spinal intradural tumor surgery in prone
position. In our study, we showed that using 5 cm H2O PEEP
perioperatively reduced the risk of pneumocephalus. However, more
cases must be studied to support this hypothesis.
(C) 2007 Lippincott Williams & Wilkins, Inc.
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Aylin
Yaman, MD, A. Osman Saatci, MD, Sulen Sarıog˘lu, MD, F.
Hakan O¨ ner, MD, Ismet Durak, MD
PURPOSE:
To determine the interaction of heavy silicone oil with various
intraocular lens (IOL) materials and whether heavy silicone oil
covers the silicone IOL optic as silicone oil does. SETTING:
Department of Ophthalmology, Dokuz Eylul University, Izmir, Turkey.
METHODS: The study group comprised 5 poly(methyl methacrylate) (PMMA)
IOLs, 4 foldable silicone IOLs, 5 foldable hydrophilic acrylic
IOLs, and 5 foldable hydrophobic acrylic IOLs. Each IOL was bathed
in balanced salt solution (BSS) for 10 minutes and then placed in
heavy silicone oil dyed with Sudan Black for another 10 minutes.
Afterward, each IOL was reimmersed in BSS for 5 minutes and
examined under the light microscope. Digital images were analyzed
to determine the optic area covered with heavy silicone oil.
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Volume 10 Issue 4 Page 454-460, June 2006
To cite this article: Mehmet Atilla Türkmen,
Salih Kavukçu, Sülen Sarıo lu,
Alper Soylu, Hülya Akhunlar, Osman Yılmaz, Hülya Güven (2006)
Effects of lactational cyclosporine A use on rat pups
Pediatric Transplantation 10 (4), 454–460.
doi:10.1111/j.1399-3046.2006.00495.x
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Abstract
Effects of lactational cyclosporine A use on rat pups
Mehmet Atilla Türkmen11Dokuz Eylül University Medical Faculty, Division of Pediatric
Nephrology, 35340, Inciralti, Izmir, Turkey, Salih Kavukçu11Dokuz Eylül University Medical
Faculty, Division of Pediatric Nephrology, 35340, Inciralti, Izmir,
Turkey, Sülen Sarıolu22Dokuz Eylül University Medical Faculty, Department of
Pathology, 35340, Inciralti, Izmir, Turkey, Alper Soylu11Dokuz Eylül University Medical
Faculty, Division of Pediatric Nephrology, 35340, Inciralti, Izmir,
Turkey, Hülya Akhunlar33Dokuz Eylül University Medical Faculty, Department of
Biochemistry, Turkey, Osman Yılmaz44Dokuz Eylül University Medical
Faculty, Animal Research Center, Izmir, Turkey and Hülya Güven55Dokuz Eylül University Medical
Faculty, Department of Pharmocology, Izmir, Turkey1Dokuz Eylül University Medical
Faculty, Division of Pediatric Nephrology, 35340, Inciralti, Izmir,
Turkey, 2Dokuz Eylül University Medical Faculty, Department of
Pathology, 35340, Inciralti, Izmir, Turkey, 3Dokuz Eylül University Medical
Faculty, Department of Biochemistry, Turkey, 4Dokuz Eylül University Medical
Faculty, Animal Research Center, Izmir, Turkey, 5Dokuz Eylül University Medical
Faculty, Department of Pharmocology, Izmir, Turkey
Abstract
Abstract: We aimed to evaluate the structural and functional changes in the thymus and kidneys of rat pups whose mothers were given cyclosporine A (CsA) during lactational period. Six adult nursing Wistar rats and their 30 pups were studied. Rat pups were divided into four groups as follows: 21-day treated group and 21-day placebo group, each including 10 breastfeeding pups sacrificed on the 21st day, whose mothers were given CsA or placebo, respectively (infancy groups) and, 60-day treated group and 60-day placebo group, each including five breastfeeding pups sacrificed on the 60th day, whose mothers were given CsA or placebo, respectively (puberty groups). While CsA levels of mother rats were very high, CsA levels of 21-day treated group pups were zero. There were no renal histomorphometric differences between study and control pups in both age groups. Renal function parameters showed significant differences between study and control pups in the infancy group: the 21-day treated group pups had significantly lower urine volume, proteinuria, FENa and urinary NAG/creatinine ratio. GFR was also lower in the 21-day treated group, but the difference was not significant, and serum creatinine levels were also not different. Renal function differences were not present among the pubertal pups. Thymic corticomedullary ratio of the 21-day treated group was significantly higher than the 21-day placebo group, while there was no difference between the 60-day treated group and 60-day placebo group. There were no significant changes in the number and distribution of CD3+, CD4+, and CD8+ thymocytes between study and control pups in both age groups. In conclusion, breastfeeding by CsA-treated mother rats induced structural alterations in the thymus and functional changes in the kidneys of the rat pups during infancy. Disturbances in the kidneys and thymus mostly improved after CsA exposure was over
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