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Comet technique:
Single cell gel electrophoresis (Single cell gel electrophoresis,
SCGE) or Comet technique, to measure DNA damage and is used to
analyze fast, simple, sensitive and widespread use is a
technical field with
Comet image is subject to change:
Molecular Genetics / Biology / Toxicology / Biyomonitoring deal
with scientists distorts DNA structure of various environmental
factors, chemical substances, such as radiation effects as a
result of the cell's DNA structure is distorted, this distortion
as a result of effects depending on the amount of queue itself as
saying that they show.
Comet parameters, with BsComet BAB Calculation
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Human eye is a seamless color has the ability to separate
20-25 tons. However, in computer technology, RGB = Kym (red,
green, blue) system according to (256x256x256) 1.6 million
tons.
According to the HSV system (360x256x256) 2.35 million
tons of looks. Each point is evaluated on its own.
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All the images found on the BAB BsComet Software comet
images automatically or semi-automatically calculates
and collects all the measurement values in a database.
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Whatever the size of the image BsComet very
rapid decomposition makes colors.
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Obtained through macro automatically analyzes the image data
base and a writer.
If more than one database in a data base with the gold.
We have determined the number of analysis will inform you.
MS excel and other data to be transferred from the database.
Some parameters calculated by BsComet'
Name, kGy, CometArea, CometLength, CometHeight,
CometIntensity, CometMeanInsety, HeadDiameter, HeadLength,
HeadArea, HeadIntensity, HeadMeanInsety, %DNAHead,
TealArea, TealIntensity, TealMeanInsety, TealLength, DNATeal,
TailMoment,OliveTailMoment,Tail Length,Comet total intensity,
TailMoment,OliveTailMoment,Tail Length, Comet total intensity,C.
total area, Tail Area, MeanIntensity
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